electroporation oor Bosnies
electroporation
en
(biotechnology) The creation of transient pores in the cell membrane, through the application of a high-voltage electrical pulse.
Vertalings in die woordeboek Engels - Bosnies
Elektroporacija
en
term in molecular biology
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Vertoon algoritmies gegenereerde vertalings
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In microbiology, the process of electroporation is often used to transform bacteria, yeast, or plant protoplasts by introducing new coding DNA.
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By the natural process of homologous recombination some of the electroporated stem cells will incorporate the new sequence with the knocked-out gene into their chromosomes in place of the original gene.
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The success of the electroporation depends greatly on the purity of the plasmid solution, especially on its salt content.
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By the 1990s, its use had escalated significantly and it is now considered a common laboratory technique, along with vesicle fusion, electroporation, chemical transfection, and viral transduction, for introducing a small amount of a substance into a small target.
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Electroporation has proven efficient for use on tissues in vivo, for in utero applications as well as in ovo transfection.
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For bacterial electroporation, typically a suspension of around 50 microliters is used.
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Adherent cells can also be transfected using electroporation, providing researchers with an alternative to trypsinizing their cells prior to transfection.
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Electroporation is performed with electroporators, purpose-built appliances which create an electrostatic field in a cell solution.
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Electroporation is also highly efficient for the introduction of foreign genes into tissue culture cells, especially mammalian cells.
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The new sequence from step 1 is introduced into the stem cells from step 2 by electroporation.
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The mixture is pipetted into the cuvette, the voltage and capacitance are set, and the cuvette is inserted into the electroporator.
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Prior to electroporation, this suspension of bacteria is mixed with the plasmid to be transformed.
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Electroporation is highly effective for transfecting cells in suspension using electroporation cuvettes.
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13 sinne gevind in 3 ms. Hulle kom uit baie bronne en word nie nagegaan nie.