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DNA melting

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The dosage of heterozygous individuals was determined using high-resolution DNA melting.
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The use of high-resolution DNA melting again allowed us to identify the dosage of Zep1 in heterozygous individuals.
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TBP is involved in DNA melting (double strand separation) by bending the DNA by 80° (the AT-rich sequence to which it binds facilitates easy melting).
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The technique is based on the fact that different parts of the DNA molecule melt at different temperatures.
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Detecting levels of sequence complexity by measuring rates of reassociation of melted DNA fragments.
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For relatively short strands of DNA, the temperature of melting is also related to strand length.
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It is postulated that ATP hydrolysis supplies the energy required for melting the DNA strands.
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In the presence of ATP, DnaA melts the DNA strands at each of these sites to form an open complex.
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In genetics, melting DNA means to separate the double-stranded DNA into two single strands by heating or the use of chemical agents, cf. polymerase chain reaction.
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Differences in base sequence cause differences in the melting properties of DNA.
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The strain imposed on the DNA through this interaction initiates melting, or separation, of the strands.
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A team of EU-funded researchers has created a cheap, easy method to 'melt' DNA (deoxyribonucleic acid) molecules into a barcode in 1 or 2 hours - instead of the usual 24.
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Dimethyl sulfate also methylates adenine in single-stranded portions of DNA (e.g., those with proteins like RNA polymerase progressively melting and re-annealing the DNA).
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(a) Melting of doubled-stranded DNA can be monitored by the absorption of ultraviolet light at 260 nm.
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The melting temperature (Tm) of DNA increases with the percentage of G∙C base pairs.
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The “melting point” of a DNA molecule is the temperature at which the double-helical strand breaks apart.
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Once again we melt the double-stranded DNA into single strands.
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You remember I said that the melting point of double-stranded DNA is about 85°C.
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The melting temperature Tm at which DNA strands will separate depends on several factors.
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In the presence of ATP, changes in DNA structure occur, which culminate in a melting reaction.
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Finally some of your RNA will melt together into strands of DNA, a more stable form because of its double helix.
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The temperature at which half the DNA has become single-stranded is known as the melting point, Tm.
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View cart “Resveratrol Malbec DNA Melting Pre-Mask” has been added to your cart.
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T m See melting profile. topoisomerase A class of enzymes that convert DNA from one topological form to another.
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The DNA is previously stained with a special fluorescent substance to get an image of the melted molecule.
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