F plasmid oor Spaans

F plasmid

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factor sexual

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F-plasmid
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The presence of the F plasmid, whether free or integrated, has important consequences for the host bacterium .
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Cells possessing a nonintegrated F plasmid are called F+.
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During excision, chromosomal genes may sometimes be incorporated into the liberated F plasmid.
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Some strains of bacteria with an integrated F-plasmid can be isolated and grown in pure culture.
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For example, the F plasmid may contain chromosomal genes (Chapter 6), in which case it is designated F¿.
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DNA replicates during transfer as for a free F plasmid (Figure 10.18).
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The Hfr chromosome breaks at the origin of transfer within the integrated F plasmid.
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When the F plasmid is integrated, chromosomal genes can be transferred along with the plasmid.
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(a) The transfer of the F plasmid converts an F- recipient cell into an F+ cell.
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The presence of the F plasmid, whether free or integrated, has important consequences for the host bacterium.
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• At which sites in the chromosome can the F plasmid integrate?
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The F plasmid is actually an episome, a plasmid that can integrate into the host chromosome.
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These provide regions of sequence homology between chromosomal and F plasmid DNA.
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This BAC contains only a small fraction of the 99.2-kbp F plasmid.
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Fplasmids are previously integrated F plasmids that have excised and captured some chromosomal genes.
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This requires an F plasmid to integrate into the chromosome to form the Hfr phenotype.
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Consists of an F plasmid with an origin of replication and the genes required to regulate replication.
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F Plasmid The F plasmid (F stands for “fertility”) is a circular DNA molecule of 99,159 bp.
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The nicking enzyme required to initiate the process, TraI, is encoded by the tra operon of the F plasmid.
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Cells that lack F plasmids are called F-negative or F-minus (F−) and as such can function as recipient cells.
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In the F-plasmid system the relaxase enzyme is called TraI and the relaxosome consists of TraI, TraY, TraM and the integrated host factor IHF.
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Among other genetic information, the F-plasmid carries a tra and trb locus, which together are about 33 kb long and consist of about 40 genes.
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A cell culture that contains in its population cells with non-integrated F-plasmids usually also contains a few cells that have accidentally integrated their plasmids.
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This occurs when the integrated plasmid (eg, F) is bracketed by two copies of an IS element.
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As you soon shall see, the F factor is in reality an autonomous genetic unit called a plasmid.
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