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Note that overall, two phosphodiester bonds were broken, but two others were formed.
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Under appropriate conditions, any particular phosphodiester bond is broken in some,but not in all, DNA molecules.
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Its close relative is the endonuclease, which cleaves phosphodiester bonds in the middle (endo) of a polynucleotide chain.
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Nucleases are enzymes that cut DNA strands by catalyzing the hydrolysis of the phosphodiester bonds.
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A phosphodiester bond joins the 3′ carbon of one nucleotide to the phosphate group of the next nucleotide.
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phosphodiester bond Chemical linkage between adjacent nucleotide residues in DNA and RNA.
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The result of this condensation reaction is called a phosphodiester linkage, or a phosphodiester bond.
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A hydrolyzing reaction that breaks phosphodiester bonds at either the 3' or the 5' end occurs.
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Also, the phosphodiester bond of the backbone is more labile when the base is U.
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Once paired, the phosphodiester bonds of the fragments can be joined by DNA ligase.
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SUMMARY n n n Phosphodiester bonds link the 3ʹ- and 5ʹ-carbons of adjacent monomers.
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This phosphate link between the sugars in adjacent nucleotides is referred to as a phosphodiester bond.
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For pTpGpTp or TGCATCA, the 5ʹ-end is at the left, and all phosphodiester bonds are 3ʹ → 5ʹ.
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Recall that DNA ligase catalyzes the formation of phosphodiester bonds between nucleotides.
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The phosphate groups in the phosphodiester bond are negatively charged.
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Under appropriate conditions, any particular phosphodiester bond is broken in some, but not in all, DNA molecules.
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DNA ligase An enzyme that can form a phosphodiester bond between the deoxyribose backbones of two strands of DNA.
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They inactivate the ribosomes by cutting a single phosphodiester bond of the rRNA that is found in a universally conserved sequence .
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When a single phosphate or two phosphates known as pyrophosphates break away and catalyze the reaction, the phosphodiester bond is formed.
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Hydrolysis of phosphodiester bonds can be catalyzed by the action of phosphodiesterases which play an important role in repairing DNA sequences.
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It is believed that the core contains common structural elements that are important for the hydrolysis of cAMP and cGMP phosphodiester bonds.
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Teichoic acids give the gram-positive cell wall an overall negative charge due to the presence of phosphodiester bonds between teichoic acid monomers.
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These ribonucleotide changes are likely to be harmful, as their ribose 2’-hydroxyl group increases susceptibility of the adjacent phosphodiester bond to hydrolysis.
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They also depurinate a single nucleotide, contiguous to the phosphodiester bond that constitutes the target of the ribotoxins, producing the same inactivating effect of the ribosome.
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In the second transesterification, the 3' end of the intron is cleaved from the downstream exon, and the two exons are joined by a phosphodiester bond.
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46 sinne gevind in 10 ms. Hulle kom uit baie bronne en word nie nagegaan nie.