propidium iodide oor Spaans

propidium iodide

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chemical compound
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compuesto químico
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Propidium iodide (PI) staining
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The brown chromosomal stain is propidium iodide and the paint is labelled with FITC.
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This is done using the fluorochromes 4,6-diamino-2-phenyl-indole (DAPI), Hoechst 33258 or propidium iodide.
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Viable cells are delineated by placing a second gate R2 selecting the population of propidium iodide-negative cells (FL3 or FL4 channel).
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The slides are then washed to remove excess unbound probe, and counterstained with 4',6-Diamidino-2-phenylindole (DAPI) or propidium iodide.
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If a counter-stain is required, each imprint may be covered with propidium iodide (0,01 mg/ml) in PBS containing 0,1 % Tween 20 and incubated for three minutes at room temperature.
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The staining procedure remains the same for each tissue, although for some tissues it may be preferable to omit the propidium iodide staining relying on phase illumination to identify the cell types present in the imprint.
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The staining procedure shall remain the same for each tissue, although for some tissues it may be preferable to omit the propidium iodide staining and rely on the phase illumination to identify the cell types present in the imprint.
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Said method comprises immunization of an Armenian hamster with neurospheres obtained from olfactory bulb cells from a 13.5-day mouse embryo and subsequent selection of the antibodies by means of neurosphere flow cytometry in the presence of propidium iodide.
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To distinguish between the two modes of cell death, apoptosis and necrosis, we examined differences in morphological and biochemical changes of cells stained with annexin V- FITC (An) and/or propidium iodide (PI) using two different assays based on flow cytometry.
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Fluorescent test chemicals can be assessed with the h-CLAT (17), nevertheless, strong fluorescent test chemicals emitting at the same wavelength as fluorescein isothiocyanate (FITC) or as propidium iodide (PI), will interfere with the flow cytometric detection and thus cannot be correctly evaluated using FITC-conjugated antibodies or PI.
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Fluorescent test chemicals can be assessed with the U-SENSTM (1), nevertheless, strong fluorescent test chemicals emitting at the same wavelength as fluorescein isothiocyanate (FITC) or as propidium iodide (PI), will interfere with the flow cytometric detection and thus cannot be correctly evaluated using FITC-conjugated antibodies (potential false negative) or PI (viability not measurable).
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Fluorescent dyes - such as propidium iodide and SYTO9 - can be used for this purpose.
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• Cell cycle analyses (Propidium Iodide).
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DNA Labeling Solution: ready-to-use solution that contains propidium iodide, permeabilization reagents and RNase.
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The control of variables was done through multi-parametric flow cytometry using Propidium Iodide and Nile Red.
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The cell cycle analysis was also performed using a flow cytometer, by fixing the cells with propidium iodide.
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No UV excitation, unlike propidium iodide.
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Viability rates were assessed by propidium iodide and flow cytometry before and after a cryopreservation cycle.
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Nuclear staining, propidium iodide (red).
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SYTO9 can permeate the membranes and thus can identify all the bacterial population, while propidium iodide is membrane-impermeable.
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The average viability rates obtained with propidium iodide was 61.89% while the mortality rate was 32.68% after a cryopreservation cycle.
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The apoptosis process of cells exposed to hydrogen peroxide was observed in real-time and analyzed by using Annexin V and propidium iodide.
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The measurement of DNA content is based on the ability of fluorescent markers such as propidium iodide (PI), for binding specifically to it.
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Ideal replacement for propidium iodide (and 7-AAD) having far better spectral properties: no UV excitation and no emission overlap with PE and homologues.
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30 sinne gevind in 9 ms. Hulle kom uit baie bronne en word nie nagegaan nie.