ligase
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ligases
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Plural form of ligase.
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In mammals a GTP-forming enzyme, succinate–CoA ligase (GDP-forming) (EC 6.2.1.4) also operates.
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This combination results in either an ADP-forming succinate-CoA ligase (A-SUCL, EC 6.2.1.5) or a GDP-forming succinate-CoA ligase (G-SUCL, EC 6.2.1.4).
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Why do bacteria have an enzyme ligase?
금속제 열쇠체인QED QED
Most organisms utilize EC 6.2.1.5, succinate–CoA ligase (ADP-forming) (despite its name, the enzyme operates in the pathway in the direction of ATP formation).
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Beginning with the lab of Paul Berg in 1972 (aided by EcoRI from Herbert Boyer's lab, building on work with ligase by Arthur Kornberg's lab), molecular biologists put these pieces together to produce the first transgenic organisms.
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So you add ligase, today, of course, if you need a ligase, how do you get it?
막대형 감초과자QED QED
ligase to appear their own breaks in DNA and, obligingly, you can purify DNA ligase.
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You place one order, you get the restriction enzymes, you get the ligases, you get the plasmids, no problem.
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And they say, well, we have an enzyme called ligase.
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Antibody to d-alanine-d-alanine ligase and detection of antibiotic-resistant bacteria using same
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Ligase will come along, join this up, and now I have a circle of DNA that has all the machinery to autonomously replicate, plus my human DNA.
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To this end, he began learning the essential tools of DNA chemistry like polymerases, ligases, gel electrophoresis, DNA synthesis etc. The fact that commercial DNA, tailor-made to specific requirements, was readily available was more than helpful for his purpose.
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12 sinne gevind in 3 ms. Hulle kom uit baie bronne en word nie nagegaan nie.