Metadata
Author: springer
Data
English[en]
MC3T3-E1 cell viability was investigated using a Wst-8 kit, and osteogenic differentiation was demonstrated by an alkaline phosphatase (ALP) activity assay. Cell attachment in CPC was observed by an environment scanning electron microscopy. Both alginate and AC microcapsules were able to release the encapsulated MC3T3-E1 cells when mixed with CPC paste.
Chinese[zh]
(1)高压静电成囊法制备载小鼠成骨前体细胞 (MC3T3-E1) 的海藻酸钙和海藻酸钠-壳聚糖微胶囊; (2)微囊化 MC3T3-E1 细胞, 进行体外培养, 使用细胞计数试剂盒(CCK-8)检测细胞活性, 并用钙黄绿素-AM(Calcein-AM) 和碘化丙啶(PI)进行活死细胞双重染色; (3)微囊化 MC3T3-E1 细胞与β-TCP/CPC 浆料复合培养后, 激光共聚焦扫描显微镜和环境扫描电子显微镜观测细胞在材料上的释放、 粘附, CCK-8 法检测材料上细胞的活力, 碱性磷酸酶 (ALP) 检测观察细胞的分化状况, 茜素红染色观察释放细胞的矿化能力。