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Researchers created a double haploid population of two such breeds, totalling 114 lines and examined the level of resistance in each.
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Of note in the 16 new CMLs are a set of five doubled-haploid lines (CML566-CML570).
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Especially valuable is the method to create double haploid lines in hybrid breeding (DH lines).
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Over 1,000 improved maize lines, including 352 doubled-haploid lines, have been developed from each cycle of the 10 biparental populations used in this study, and tested in multi-location trials.
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During the visit to the CIMMYT-Kenya Agricultural and Livestock Research Organization (KALRO) experimental station at Kiboko, participants experienced all the processes involved in maize doubled haploid line production and the development of high-yielding maize varieties with tolerance to drought and low nitrogen.
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It is estimated that about 70 to 80 percent of new maize hybrids being produced currently by major seed companies in the world, especially in North America and Europe, contain one or more doubled-haploid lines, with DH-based maize hybrids covering about 40 to 50 million hectares worldwide.
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As complementary activities, clonal multiplication and micropropagation techniques of selected materials are applied, as well as generation of double haploid and polyploid lines in different species.
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Lumpkin singled out several technologies in use or in the pipeline that will increase yields and help mitigate the effects of climate change, water shortages and disease epidemics: • Doubled haploid maize inducer lines developed for the tropics.
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Alba Arcos, a CIMMYT-Colombia PhD student, presented on doubled haploid technology and how this can be harnessed to accelerate inbred line development combining different favorable traits.
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Pulso colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and prediction and inbreeding of maize synthetics generated with lines and single crosses.
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They are called pure lines. Some biotechnological techniques based on in vitro culture, can accelerate the production of pure lines through the production of double haploid plants in only one in vitro generation. This approach allows for a significant reduction of the time and resources needed.
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Fixed line: Denotes a plant genotype that is characterized by homozygosity in most loci, ideally a so-called double haploid.
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The training program also included a field visit to maize research station at Kiboko, and the maize doubled-haploid facility at the Kenya Agricultural and Livestock Research Organization to evaluate maize inbred lines and hybrids planted for genetic purity demonstrations.
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He added that the resistance would be speedily incorporated into breeding lines and populations through screening at the MLN facility in Naivasha and use of the doubled haploid facility in Kiboko, both inaugurated in March 2013.
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