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fluorescent antibody technique

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inmunofluorescencia

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inmunoensayo fluorescente

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método de inmunofluorescencia

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técnicas de inmunofluorescencia

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An immunological staining procedure such as the fluorescent antibody technique (FA) or an enzyme-linked antibody test e. g. immunoperoxidase (IPX) are used to detect BVD virus in inocuiated cultures.
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A total of 2,096 serum and tissue samples were tested for IgM antibodies against yellow fever by capture enzyme-linked immunosorbent assay, viral isolation-indirect fluorescence antibody technique, and reverse transcriptase-polymerase chain reaction.
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An indirect fluorescent antibody (IFA) technique has been developed for detection of anti-Dermatophilus antibodies in sheep.
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The sera of two groups of cattle, each infected with one of these parasites, were studied by the indirect fluorescent antibody technique, using both standardTheileria parva andT. mutans antigens and antigens prepared from the infective parasites under study.
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Studies in Rossmoyne silt loam by the plate count (PC) and fluorescent antibody (FA) techniques showed that flooding had no detrimental effects on survival and N2-fixing capacity ofR. phaseoli strains.
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The indirect fluorescent antibody test is an important technique to this end.
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Keywords: Leishmania (L.) infantum chagasi; Infection; Immunologic Tests; Hypersensitivity, Delayed; Fluorescent Antibody Technique, Indirect.
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Palavras-chave: Leishmaniasis [Visceral]; Leishmaniasis [diagnosis]; Dogs; Fluorescent Antibody Technique; Diagnostic Techniques and Procedures; Sensitivity and Specificity; Seroepidemiologic Studies.
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The consortium employed innovative imaging techniques such as PET and near-infrared fluorescence imaging to address antibody biodistribution.
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Other diagnostic techniques of proven similar efficacy such as Indirect fluorescent antibody test (IFAT), Enzyme-linked immuno-sorbent assay (ELISA), RT-PCR and Immunohistochemistry (IHC).
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Public Health, Communicable Diseases, Parasitic Diseases, Parasitic Diseases, Animal, Diagnostic Techniques and Procedures, Leishmaniasis, Leishmania, Trypanosoma cruzi, Fluorescent Antibody Technique, Fluorescent Antibody Technique, Indirect, Cross Reactions, Dogs
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The research work performed also used a novel technique of local UV-irradiation combined with fluorescent antibody labelling for studying the assembly of the nucleotide excision repair (NER) complex in normal and repair-deficient (xeroderma pigmentosum) human cells.
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The sensitivity of the eye to light is increased during dark adaptation.Fluorescent Antibody Technique, Indirect: A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases.
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In contrast with HARES, rabbits have 22 chromosome pairs.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT).
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It was developed by the Sprague-Dawley Animal Company.Spectroscopy, Fourier Transform Infrared: A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.Fluorescent Antibody Technique, Indirect: A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases.
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It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT).
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The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.Spleen: An encapsulated lymphatic organ through which venous blood filters.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT).
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They are mainly involved in blood coagulation.Fluorescent Antibody Technique, Direct: A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)Antigens, Fungal: Substances of fungal origin that have antigenic activity.Bacterial Proteins: Proteins found in any species of bacterium.Antigens, CD4: 55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types.
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Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT).
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Certain staining techniques cause components of the ribosomes to precipitate into characteristic "reticulum" (not the same as the ENDOPLASMIC RETICULUM), hence the name reticulocytes.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers.
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The tissue is then examined by fluorescence microscopy.Immunoassay: A technique using antibodies for identifying or quantifying a substance.
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The population is usually patients with a specific disease but the concept is not restricted to humans and is not restricted to medicine.Geographic Atrophy: A form of MACULAR DEGENERATION also known as dry macular degeneration marked by occurrence of a well-defined progressive lesion or atrophy in the central part of the RETINA called the MACULA LUTEA. It is distinguishable from WET MACULAR DEGENERATION in that the latter involves neovascular exudates.Fluorescent Antibody Technique, Indirect: A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases.
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A distinct elliptical precipitin arc results for each protein detectable by the antisera.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Autoantibodies: Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.Epitopes: Sites on an antigen that interact with specific antibodies.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain.
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In this technique, samples are labelled directly with fluorescent antibodies to visualise nucleocapsids.
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