lipofection oor Spaans

lipofection

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en
liposome transfection

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lipofección

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Two other procedures to transfect cells are electroporation and lipofection.
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described. For human cancer melanoma cells, the treatment leads to a very high perforation rate of 70%, transfection efficiency three times higher than for conventional lipofection, and very low toxicity (<1%).
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Another method by which naked nucleic acid can be introduced into cells includes liposome-mediated transfection (lipofection).
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No differences were found in gene targeting efficiency between groups; however, lipofection was three times more efficient than electroporation in transfection efficiency, which makes lipofection a less expensive alternative method to produce gene targeting in mouse embryonic stem cells.
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Portions stained red Figure 9A: A figure showing the results obtained by transiently transfecting each mutant gene hdlk-1 described in COS-7 cells by lipofection and then performing FACS analysis on the cells 24 to 72 hours after gene transfection.
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Microelectroporation 23 is an in vitro transfection method that has been recently adopted by many researchers and it has a high homologous recombination efficiency (10-1); however, this method still has the disadvantage of requiring sophisticated and delicate equipment and highly trained personnel, which still leaves lipofection as simple and cost effective transfection method to produce transgenic gene targeted mice.
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17 Lipofection was performed with LipofectA- MINE ** following manufacturer s recommendations.
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We have analyzed the in vitro sensitivity to the IFNβ gene lipofection in three human cutaneous and five canine mucosal melanoma cells lines.
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In this technique the transgene gains entrance to the cell through an endocytosis like process, where the transgene encapsulating liposomes, in aqueous solution, find an oppositely charged specific receptor bipolymer from the cell membrane that interacts with the microtubules Lipofection has been successfully used to produce random integration in pig fetal fibroblasts, 11 gene targeting in sheep fibroblasts, 12 to transfect mes cells for transient expression of genes, 7 to produce random integration of transgenes, 13,14 to transfer interference RNA 15 and to produce targeted mutations with oligonucleotides.
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The objective of this work was to find out if lipofection can be used as efficiently as electroporation for regular gene targeting protocols. This context compares gene targeting efficiency between these techniques in mouse embryonic stem cells E14TG2a, using a gene replacement type vector.
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It has been shown that after lipofection, mouse embryonic stem cells remain pluripotent, capable of forming germ line chimeras and can be transfected with greater efficiency than with electroporation; however, gene targeting of mouse embryonic stem cells by lipofection has not been reported.
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Lipofection is a transfection technique with a high cell survival rate.
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Non-virus systems contain the injection of naked DNA in form of a plasmid or the lipofection, the injection of liposomes which carries the DNA, further the electroporation or nucleofection – the direct injection of DNA in the cell nucleus.
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Then, the genetic material of this new vector (now identified as “pIRES-hLf-hLs/Neo”) was introduced into the fibroblasts by lipofection and after a period of cell culture and selection, resistant cells were obtained, “that is, cells having the vector”, he said.
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Lipofection is a transfection method that requires lower amounts of cells and DNA than electroporation, and has proven to be efficient in a large number of cell lines.
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It has been shown that after lipofection, mouse embryon (mas) ic stem cells remain pluripotent, capable of forming germ line chimeras and can be transfected with greater efficiency than with electroporation; however, gene targeting of mouse embryonic stem cells by lipofection has not been reported.
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