Approximate confidence limits may be obtained with standard non-linear regression programs with re-parameterisation (Bruce and Versteeg, #), which involves rewriting the mathematical equation with the desired point estimates, e.g. the EC# and the EC# as the parameters to be estimated. (Let the function be I = f (α, β, concentration) and utilise the definition relationships f (α, β, EC#) = #,# and f (α, β, EC#) = #,# to substitute f (α, β, concentration) with an equivalent function g (EC#, EC#, concentration
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