The present invention relates to novel beta-galactosidase and, more specifically, to novel beta-galactosidase derived from Bacillus circulans, a gene encoding the same, a recombinant vector and a recombinant microorganism containing the gene, a method for preparing beta-galactosidase using the recombinant microorganism, and a method for preparing galactooligosaccharide using the beta-galactosidase.
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